David Barclay served as animatronic effects supervisor. Puppeteers included Adam Behr, Kevin Carlson, Alejandro Diaz, Randi Kaplan, John Kennedy, Luke Khanlian, Bruce Lanoil (lead performer for Mr. Tinkles), Drew Massey, Gord Robertson, Michelan Sisti, and Allan Trautman (lead puppeteer for Calico). Marian Keating and Julie Zobel were senior fabricators, and Rob Hinderstein supplied eyes, teeth, and tongues.
The 2010 sequel, Cats & Dogs: The Revenge of Kitty Galore, did not have involvement of the Creature Shop. Instead, Lee Romaire Studios provided the creatures. David Barclay was once again the animatronic effects supervisor.
The film begins with the SUV of the Brody family pulling up to the house, with Mrs. Brody (Elizabeth Perkins) and her son, Scott Brody (Alexander Pollock), bringing in the groceries, and the family's pet bloodhound, Buddy, immediately starts chasing a cat.
Buddy follows the cat and pursues him through a neighbor's house, until he finds the cat lying dead on the street, obviously playing dead. The cat gets up and runs away, and a blue van pulls up and captures Buddy.
An Anatolian Shepherd Dog named Butch (Alec Baldwin) watches from a window, then goes to his doghouse, pressing a blue button, which grants him access to a large techno-network, and tells an agent of Buddy's kidnap. At Intel HQ, the head dog receives the news about Buddy and orders the best agents to accomplish the mission and defeat the cat menace.
Meanwhile, at a farm, a small group of beagle puppies converse and make fun of a younger puppy (Tobey Maguire), who wants to be free. A group of young black Doberman puppies led by a large Doberman Pinscher force the beagles to go underground as Mrs. Brody approaches. Mrs. Brody comes into the puppy pen, and the last beagle puppy comes out. She decides to adopt him and takes him home, naming him "Lou". She puts him outside to play and a golden bone attached to a balloon falls down. Lou goes near it, and Butch comes out and throws a stick at it, making it explode.
Butch then takes Lou into Buddy's doghouse, and shows him the network that dog agents use, and takes him to meet some more agents: Peek (Joe Pantoliano) is a Chinese Crested Dog, who works in an underground tube and has computers, radar, sonar, television, communications, satellite, thermal imaging systems, security cameras, and such. Sam (Michael Clarke Duncan) is a comical Bearded Collie.
Meanwhile, Mr. Tinkles (Sean Hayes), a white Persian cat and the main antagonist, plans to conquer the world by making all humans allergic to dogs with Mr. Brody (Jeff Goldblum)'s research on a cure for dog allergies.
Back in the Brody family's backyard, Lou needs to be named for a secret agent. He was thinking "total inielation." But that name was taken. But he wanted to be "Doom Machine." But Butch wanted to call himself "Slinky the Space Dog." He was still not an agent but he is gonna help them.
- Jeff Goldblum as Professor Brody
- Elizabeth Perkins as Mrs. Brody
- Alexander Pollock as Scotty Brody
- Miriam Margolyes as Sophie
- Tobey Maguire as Lou (voice)
- Alec Baldwin as Butch (voice)
- Sean Hayes as Mr. Tinkles (voice)
- Susan Sarandon as Ivy (voice)
- Joe Pantoliano as Peek (voice)
- Michael Clarke Duncan as Sam (voice)
- Jon Lovitz as Calico (voice)
- Myron Natwick as Mr. Mason
- Victor Wilson as Doberman Drill Sergeant (voice)
- Salome Jens as Collie at HQ (voice)
- Charlton Heston as Mastiff (voice)
- Glenn Ficarra as The Russian (voice)
- Danny Mann and Billy West as The Ninjas (voice)
- Paul Pape as Wolf Blitzer (voice)