While normally mice were popular prey and food for cats, Mr. Tinkles enlisted a number of them (male and female) to help in his plan to spread the dog allergen throughout the world, having some of them sneak into humans' homes, and others infiltrate ships and boats in the first movie. When the plan failed, the mice were arrested by the dogs. In the sequel, the main villain, Kitty Galore had a pet mouse named Scrumptious who eventually turned against her due to her treatment of him.

Note: Those mice is the similar to the mice characters (such as Roddy St. James) from 2006's Flushed Away.

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